An investigation of nitrile transforming enzymes in the chemo-enzymatic synthesis of the taxol sidechain

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00448779" target="_blank" >RIV/61388971:_____/15:00448779 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/c5ob01191d" target="_blank" >http://dx.doi.org/10.1039/c5ob01191d</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c5ob01191d" target="_blank" >10.1039/c5ob01191d</a>

Result language
angličtina
Original language name
An investigation of nitrile transforming enzymes in the chemo-enzymatic synthesis of the taxol sidechain
Original language description
Paclitaxel (taxol) is an antimicrotubule agent widely used in the treatment of cancer. Taxol is prepared in a semisynthetic route by coupling the N-benzoyl-(2R, 3S)-3-phenylisoserine sidechain to the baccatin III core structure. Precursors of the taxol sidechain have previously been prepared in chemoenzymatic approaches using acylases, lipases, and reductases, mostly featuring the enantioselective, enzymatic step early in the reaction pathway. Here, nitrile hydrolysing enzymes, namely nitrile hydratasesand nitrilases, are investigated for the enzymatic hydrolysis of two different sidechain precursors. Both sidechain precursors, an openchain alpha-hydroxy-beta-amino nitrile and a cyanodihydrooxazole, are suitable for coupling to baccatin III directly after the enzymatic step. An extensive set of nitrilases and nitrile hydratases was screened towards their activity and selectivity in the hydrolysis of two taxol sidechain precursors and their epimers. A number of nitrilases and nitrile h
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Project
<a href="/en/project/GAP504%2F11%2F0394" target="_blank" >GAP504/11/0394: Interspecies comparison of nitrilases by using recombinant enzymes obtained by database-mining: their environmental impact and potential application</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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