Upscale of recombinant alpha-L-rhamnosidase production by Pichia pastoris Mut(S) strain

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00450837" target="_blank" >RIV/61388971:_____/15:00450837 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3389/fmicb.2015.01140" target="_blank" >http://dx.doi.org/10.3389/fmicb.2015.01140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2015.01140" target="_blank" >10.3389/fmicb.2015.01140</a>

Result language

angličtina

Original language name

Upscale of recombinant alpha-L-rhamnosidase production by Pichia pastoris Mut(S) strain

Original language description

Pichia pastoris is currently one of the most preferred microorganisms for recombinant enzyme production due to its efficient expression system. The advantages include the production of high amounts of recombinant proteins containing the appropriate posttranslational modifications and easy cultivation conditions. alpha-L-Rhamnosidase is a biotechnologically important enzyme in food and pharmaceutical industry, used for example in debittering of citrus fruit juices, rhamnose pruning from naringin, or enhancement of wine aromas, creating a demand for the production of an active and stable enzyme. The production of recombinant alpha-L-rhamnosidase cloned in the Mut(S) strain of P pastoris KM71H was optimized. The encoding gene is located under the controlof the AOX promoter, which is induced by methanol whose concentration is instrumental for these strain types. Fermentation was upscaled in bioreactors employing various media and several methanol-feeding strategies. It was found that fed

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

OCT 2015

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

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UT code for WoS article

000363861000001

EID of the result in the Scopus database

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