Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00422632" target="_blank" >RIV/61388971:_____/13:00422632 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.biortech.2013.08.083" target="_blank" >http://dx.doi.org/10.1016/j.biortech.2013.08.083</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biortech.2013.08.083" target="_blank" >10.1016/j.biortech.2013.08.083</a>

Result language

angličtina

Original language name

Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus

Original language description

This study describes an efficient, large scale fermentation of a recombinant alpha-L-rhamnosidase originating from Aspergillus terreus. High-cell-density Pichia pastoris fermentation resulted in yields up to 627 U/L/h. The recombinant enzyme was used forthe reverse rhamnosylation of various small organic compounds. A full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the reaction mixture. Careful optimization of these parameters allowed the synthesis of a range of alpha-L-rhamnosides among which cyclohexyl alpha-L-rhamnopyranoside, anisyl alpha-L-rhamnopyranoside and 2-phenylethyl alpha-L-rhamnopyranoside. In addition, alpha-L-rhamnosylation of phenolic hydroxyls in phenols such as hydroquinone, resorcinol, catechol and phenol was observed, which is a rather unique reaction catalyzed by glycosidases

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioresource Technology

ISSN

0960-8524

e-ISSN

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Volume of the periodical

147

Issue of the periodical within the volume

NOV 2013

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

640-644

UT code for WoS article

000326660900088

EID of the result in the Scopus database

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