Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-alpha-l-rhamnosyl-beta-d-glucosidase active on flavonoids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00460161" target="_blank" >RIV/61388971:_____/16:00460161 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00253-015-7088-x" target="_blank" >http://dx.doi.org/10.1007/s00253-015-7088-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00253-015-7088-x" target="_blank" >10.1007/s00253-015-7088-x</a>
Alternative languages
Result language
angličtina
Original language name
Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-alpha-l-rhamnosyl-beta-d-glucosidase active on flavonoids
Original language description
Bacteria represent an underexplored source of diglycosidases. Twenty-five bacterial strains from the genera Actinoplanes, Bacillus, Corynebacterium, Microbacterium, and Streptomyces were selected for their ability to grow in diglycosylated flavonoids-based media. The strains Actinoplanes missouriensis and Actinoplanes liguriae exhibited hesperidin deglycosylation activity (6-O-alpha-L-rhamnosyl-beta-D-glucosidase activity, EC 3.2.1.168), which was 3 to 4 orders of magnitude higher than the corresponding monoglycosidase activities. The diglycosidase production was confirmed in A. missouriensis by zymographic assays and NMR analysis of the released disaccharide, rutinose. The gene encoding the 6-O-alpha-L-rhamnosyl-beta-D-glucosidase was identified in the genome sequence of A. missouriensis 431(T) (GenBank accession number BAL86042.1) and functionally expressed in Escherichia coli. The recombinant protein hydrolyzed hesperidin and hesperidin methylchalcone, but not rutin, which indicates its specificity for 7-O-rutinosylated flavonoids.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Microbiology and Biotechnology
ISSN
0175-7598
e-ISSN
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Volume of the periodical
100
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
3061-3070
UT code for WoS article
000372282000011
EID of the result in the Scopus database
2-s2.0-84960813852