Cyanide hydratases and cyanide dihydratases: emerging tools in the biodegradation and biodetection of cyanide

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00451731" target="_blank" >RIV/61388971:_____/15:00451731 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/15:10297017

Result on the web

<a href="http://dx.doi.org/10.1007/s00253-015-6899-0" target="_blank" >http://dx.doi.org/10.1007/s00253-015-6899-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-015-6899-0" target="_blank" >10.1007/s00253-015-6899-0</a>

Result language

angličtina

Original language name

Cyanide hydratases and cyanide dihydratases: emerging tools in the biodegradation and biodetection of cyanide

Original language description

The purpose of this study is to summarize the current knowledge of the enzymes which are involved in the hydrolysis of cyanide, i.e., cyanide hydratases (CHTs; EC 4.2.1.66) and cyanide dihydratases (CynD; EC 3.5.5.1). CHTs are probably exclusively produced by filamentous fungi and widely occur in these organisms; in contrast, CynDs were only found in a few bacterial genera. CHTs differ from CynDs in their reaction products (formamide vs. formic acid and ammonia, respectively). Several CHTs were also found to transform nitriles but with lower relative activities compared to HCN. Mutants of CynDs and CHTs were constructed to study the structure-activity relationships in these enzymes or to improve their catalytic properties. The effect of the C-terminal part of the protein on the enzyme activity was determined by constructing the corresponding deletion mutants. CynDs are less active at alkaline pH than CHTs. To improve its bioremediation potential, CynD from Bacillus pumilus was engineered by directed evolution combined with site-directed mutagenesis, and its operation at pH 10 was thus enabled. Some of the enzymes have been tested for their potential to eliminate cyanide from cyanide-containing wastewaters. CynDs were also used to construct cyanide biosensors

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

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Volume of the periodical

99

Issue of the periodical within the volume

21

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

8875-8882

UT code for WoS article

000363481400005

EID of the result in the Scopus database

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