Biotransformation of free cyanide to formic acid by a cyanide hydratase-formamidase cascade reaction
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00587363" target="_blank" >RIV/61388971:_____/24:00587363 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/24:00382234 RIV/00216208:11310/24:10495586 RIV/60461373:22330/24:43929721
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1359511324001041?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1359511324001041?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.procbio.2024.04.009" target="_blank" >10.1016/j.procbio.2024.04.009</a>
Alternative languages
Result language
angličtina
Original language name
Biotransformation of free cyanide to formic acid by a cyanide hydratase-formamidase cascade reaction
Original language description
Free cyanide (fCN) consisting of HCN and CN is highly hazardous. Today, removal of cyanide from industrial (mining, plating, cokeplant) wastewaters largely relies on physicochemical processes followed by microbial degradation. Enzymatic processes are gaining ground but are still at a low technological stage. The cyanideconverting enzymes of interest are primarily cyanide dihydratases (CynDs, EC 3.5.5.1.), which hydrolyze HCN to formic acid and ammonia, and cyanide hydratases (CynHs, EC 4.2.1.66), which hydrate HCN to formamide. CynHs usually have much higher specific activities and a broader pH profile especially in the alkaline region compared to CynDs. However, the product of CynH, formamide, although much less toxic than fCN, still poses a significant health risk. Therefore, it is attractive to combine the CynH with an amidase that converts formamide to formic acid and ammonia. Here we demonstrate on a laboratory scale a two-step ´onepot´ detoxification of fCN (5 mM) to formic acid using recombinantly produced purified enzymes CynH from Exidia glandulosa and formamidase (EC 3.5.1.49) from Bacillus cereus . The reaction proceeds at pH 9.0-10.0, which reduces the risk of HCN escape. We also hypothesize that the cascade can be used for fCN determination after coupling an NADdependent formate dehydrogenase.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA22-06785S" target="_blank" >GA22-06785S: Free-cyanide determination: new approaches based on enzyme cascades</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Process Biochemistry
ISSN
1359-5113
e-ISSN
1873-3298
Volume of the periodical
142
Issue of the periodical within the volume
July 2024
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
62-67
UT code for WoS article
001235474900001
EID of the result in the Scopus database
2-s2.0-85190269334