Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00549581" target="_blank" >RIV/61388971:_____/21:00549581 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/21:00354175
Result on the web
<a href="https://www.mdpi.com/2073-4344/11/11/1410" target="_blank" >https://www.mdpi.com/2073-4344/11/11/1410</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/catal11111410" target="_blank" >10.3390/catal11111410</a>
Alternative languages
Result language
angličtina
Original language name
Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents
Original language description
Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN-). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from Exidia glandulosa (protein KZV92691.1 designated NitEg by us), which was overproduced in Escherichia coli. The purified NitEg was highly active for fCN with 784 U/mg protein, k(cat) 927/s and k(cat)/K-M 42/s/mM. It exhibited optimal activities at pH approximately 6-9 and 40-45 & DEG,C. It was quite stable in this pH range, and retained approximately 40% activity at 37 & DEG,C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30-40%. The removal of 98-100% fCN was achieved for 0.6-100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA18-00184S" target="_blank" >GA18-00184S: New "nitrilase superfamily" proteins in Basidiomycota: study of their activities and potential functions in the biodegradation of cyanide and nitriles</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Catalysts
ISSN
2073-4344
e-ISSN
2073-4344
Volume of the periodical
11
Issue of the periodical within the volume
11
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
1410
UT code for WoS article
000723298100001
EID of the result in the Scopus database
2-s2.0-85119371203