Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00451983" target="_blank" >RIV/61388971:_____/15:00451983 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/15:43909252

Result on the web

<a href="http://dx.doi.org/10.1016/j.yexcr.2014.12.005" target="_blank" >http://dx.doi.org/10.1016/j.yexcr.2014.12.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.yexcr.2014.12.005" target="_blank" >10.1016/j.yexcr.2014.12.005</a>

Result language

angličtina

Original language name

Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel

Original language description

Resistance of cancer cells to chemotherapeutic agents is one of the main causes of treatment failure. In order to detect proteins potentially involved in the mechanism of resistance to taxanes, we assessed differences in protein expression in MCF-7 breast cancer cells that are sensitive to paclitaxel and in the same cells with acquired resistance to paclitaxel (established in our lab). Proteins were separated using two-dimensional electrophoresis. Changes in their expression were determined and proteinswith altered expression were identified using mass spectrometry. Changes in their expression were confirmed using western blot analysis. With these techniques, we found three proteins expressed differently in resistant MCF-7 cells, i.e., thyroid hormone-interacting protein 6 (TRIP6; upregulated to 650%), heat shock protein 27 (HSP27; downregulated to 50%) and cathepsin D (downregulated to 28%). Silencing of TRIP6 expression by specific siRNA leads to decreased number of grown resistant

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/NT13679" target="_blank" >NT13679: Study of mechanism of action of biomarkers correlating with breast cancer therapy outcome</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental Cell Research

ISSN

0014-4827

e-ISSN

—

Volume of the periodical

333

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000352823600001

EID of the result in the Scopus database

—