Genetic transformation of extremophilic fungi Acidea extrema and Acidothrix acidophila

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00452244" target="_blank" >RIV/61388971:_____/15:00452244 - isvavai.cz</a>

Alternative codes found

RIV/44555601:13440/15:43886672

Result on the web

<a href="http://dx.doi.org/10.1007/s12223-015-0398-7" target="_blank" >http://dx.doi.org/10.1007/s12223-015-0398-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-015-0398-7" target="_blank" >10.1007/s12223-015-0398-7</a>

Result language

angličtina

Original language name

Genetic transformation of extremophilic fungi Acidea extrema and Acidothrix acidophila

Original language description

Intact, growing cells of strongly acidophilic fungi Acidea extrema and Acidothrix acidophila have been successfully transformed by introduction of heterologous DNA fragment (composed of the glyceraldehyde-phosphate-dehydrogenase gene promoter from Emericella nidulans, a metallothionein-coding gene AsMt1 from Amanita strobiliformis and glyceraldehyde-phosphate-dehydrogenase gene terminator from Colletotrichum gloeosporioides) with the length of 1690 bp. The transformation procedure was based on the DNA transfer mediated by Agrobacterium tumefaciens bearing disarmed helper plasmid pMP90 and binary vector pCambia1300 with inserted DNA fragment of interest. The transformants proved to be mitotically stable, and the introduced gene was expressed at least atthe level of transcription. Our work confirms that metabolic adaptations of strongly acidophilic fungi do not represent an obstacle for genetic transformation using conventional methods and can be potentially used for production of heter

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GAP504%2F11%2F0484" target="_blank" >GAP504/11/0484: Hyperaccumulation of heavy metals in macrofungi - molecular, geomycological and ecotaxonomic aspects</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

60

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

365-371

UT code for WoS article

000355216300011

EID of the result in the Scopus database

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