Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00461200" target="_blank" >RIV/61388971:_____/16:00461200 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/16:00461200 RIV/00216208:11310/16:10323877 RIV/00216208:11320/16:10323877
Result on the web
<a href="http://nar.oxfordjournals.org/content/44/7/3000.full" target="_blank" >http://nar.oxfordjournals.org/content/44/7/3000.full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkw171" target="_blank" >10.1093/nar/gkw171</a>

Result language
angličtina
Original language name
Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases
Original language description
DNA templates containing a set of base modifications in the major groove (5-substituted pyrimidines or 7-substituted 7-deazapurines bearing H, methyl, vinyl, ethynyl or phenyl groups) were prepared by PCR using the corresponding base-modified 2'-deoxyribonucleoside triphosphates (dNTPs). The modified templates were used in an in vitro transcription assay using RNA polymerase from Bacillus subtilis and Escherichia coli. Some modified nucleobases bearing smaller modifications (H, Me in 7-deazapurines) were perfectly tolerated by both enzymes, whereas bulky modifications (Ph at any nucleobase) and, surprisingly, uracil blocked transcription. Some middle-sized modifications (vinyl or ethynyl) were partly tolerated mostly by the E. coli enzyme. In all cases where the transcription proceeded, full length RNA product with correct sequence was obtained indicating that the modifications of the template are not mutagenic and the inhibition is probably at the stage of initiation. The results are promising for the development of bioorthogonal reactions for artificial chemical switching of the transcription.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/GA14-04289S" target="_blank" >GA14-04289S: Modifications and bioorthogonal reactions in the major groove of DNA for regulation of protein binding and gene expression</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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