Bacteriophage-related epigenetic natural and non-natural pyrimidine nucleotides and their influence on transcription with T7 RNA polymerase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00600677" target="_blank" >RIV/61388963:_____/24:00600677 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10494953
Result on the web
<a href="https://doi.org/10.1038/s42004-024-01354-5" target="_blank" >https://doi.org/10.1038/s42004-024-01354-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s42004-024-01354-5" target="_blank" >10.1038/s42004-024-01354-5</a>
Alternative languages
Result language
angličtina
Original language name
Bacteriophage-related epigenetic natural and non-natural pyrimidine nucleotides and their influence on transcription with T7 RNA polymerase
Original language description
DNA modifications on pyrimidine nucleobases play diverse roles in biology such as protection of bacteriophage DNA from enzymatic cleavage, however, their role in the regulation of transcription is underexplored. We have designed and synthesized a series of uracil 2ʹ-deoxyribonucleosides and 5ʹ-O-triphosphates (dNTPs) bearing diverse modifications at position 5 of nucleobase, including natural nucleotides occurring in bacteriophages, α-putrescinylthymine, α-glutaminylthymine, 5-dihydroxypentyluracil, and methylated or non-methylated 5-aminomethyluracil, and non-natural 5-sulfanylmethyl- and 5-cyanomethyluracil. The dNTPs bearing basic substituents were moderate to poor substrates for DNA polymerases, but still useful in primer extension synthesis of modified DNA. Together with previously reported epigenetic pyrimidine nucleotides, they were used for the synthesis of diverse DNA templates containing a T7 promoter modified in the sense, antisense or in both strands. A systematic study of the in vitro transcription with T7 RNA polymerase showed a moderate positive effect of most of the uracil modifications in the non-template strand and some either positive or negative influence of modifications in the template strand. The most interesting modification was the non-natural 5-cyanomethyluracil which showed significant positive effect in transcription.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA for therapy</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Communications Chemistry
ISSN
2399-3669
e-ISSN
2399-3669
Volume of the periodical
7
Issue of the periodical within the volume
November
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
256
UT code for WoS article
001351596800002
EID of the result in the Scopus database
2-s2.0-85209818120