Homologues of epigenetic pyrimidines: 5-alkyl-, 5-hydroxyalkyl and 5-acyluracil andcytosine nucleotides: synthesis, enzymatic incorporation into DNA and effect on transcription with bacterial RNA polymerase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00559506" target="_blank" >RIV/61388963:_____/22:00559506 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/22:00559506 RIV/00216208:11310/22:10454619
Result on the web
<a href="https://doi.org/10.1039/D2CB00133K" target="_blank" >https://doi.org/10.1039/D2CB00133K</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d2cb00133k" target="_blank" >10.1039/d2cb00133k</a>
Alternative languages
Result language
angličtina
Original language name
Homologues of epigenetic pyrimidines: 5-alkyl-, 5-hydroxyalkyl and 5-acyluracil andcytosine nucleotides: synthesis, enzymatic incorporation into DNA and effect on transcription with bacterial RNA polymerase
Original language description
Homologues of natural epigenetic pyrimidine nucleosides and nucleotides were designed and synthesized. They included 5-ethyl-, 5-propyl-, 5-(1-hydroxyethyl)-, 5-(1-hydroxypropyl)- and 5-acetyl- and 5-propionyl-cytosine anduracil 2'-deoxyribonucleosides and their corresponding 5'-O-triphosphates (dN(x)TPs). The epimers of 5-(1-hydroxyethyl)- and 5-(1-hydroxypropyl)pyrimidine nucleosides were separated and their absolute configuration was determined by a combination of X-ray and NMR analysis. The modified dN(x)TPs were used as substrates for PCR synthesis of modified DNA templates used for the study of transcription with bacterial RNA polymerase. Fundamental differences in transcription efficiency were observed, depending on the various modifications. The most notable effects included pronounced stimulation of transcription from 5-ethyluracil-bearing templates (200% transcription yield compared to natural thymine) and an enhancing effect of 5-acetylcytosine versus inhibiting effect of 5-acetyluracil. In summary, these results reveal that RNA polymerase copes with dramatically altered DNA structure and suggest that these nucleobases could potentially play roles as artificial epigenetic DNA nucleobases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RSC Chemical Biology
ISSN
2633-0679
e-ISSN
2633-0679
Volume of the periodical
3
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
1069-1075
UT code for WoS article
000825796800001
EID of the result in the Scopus database
2-s2.0-85134389118