Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00462752" target="_blank" >RIV/61388971:_____/16:00462752 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00284-016-1077-x" target="_blank" >http://dx.doi.org/10.1007/s00284-016-1077-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00284-016-1077-x" target="_blank" >10.1007/s00284-016-1077-x</a>
Alternative languages
Result language
angličtina
Original language name
Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors
Original language description
Promoter activities in Corynebacterium glutamicum strains with deletions of genes encoding sigma factors of RNA polymerase suggested that transcription from some promoters is controlled by two sigma factors. To prove that different sigma factors are involved in the recognition of selected Corynebacterium glutamicum promoters, in vitro transcription system was applied. It was found that a typical housekeeping promoter Pper interacts with the alternative sigma factor sigma(B) in addition to the primary sigma factor sigma(A). On the other way round, the sigma(B)-dependent promoter of the pqo gene that is expressed mainly in the stationary growth phase was active also with sigma(A). Some promoters of genes involved in stress responses (P1clgR, P2dnaK, and P2dnaJ2) were found to be recognized by two stress-responding sigma factors, sigma(H) and sigma(E).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Microbiology
ISSN
0343-8651
e-ISSN
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Volume of the periodical
73
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
401-408
UT code for WoS article
000381114200015
EID of the result in the Scopus database
2-s2.0-84982792270