Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00476299" target="_blank" >RIV/61388971:_____/17:00476299 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/s13568-017-0436-8" target="_blank" >http://dx.doi.org/10.1186/s13568-017-0436-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13568-017-0436-8" target="_blank" >10.1186/s13568-017-0436-8</a>

Result language

angličtina

Original language name

Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum

Original language description

Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (sA), the primary-like sigma(B) and five alternative sigma factors (sigma(C), sigma(D), sigma(E), sigma(H) and sigma(M)). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters. To document the strengths of these methods, we tested examples of respective promoters for each C. glutamicum sigma factor. Promoters of the rshA (anti-sigma for sH) and trxB1 (thioredoxin) genes were found to be sigma(H)-dependent, whereas the promoter of the sigB gene (sigma factor sigma(B)) was sigma(E)-and sigma(H)-dependent. It was confirmed that the promoter of the cg2556 gene (iron-regulated membrane protein) is sigma(C)-dependent as suggested recently by other authors. The promoter of cmt1 (trehalose corynemycolyl transferase) was found to be clearly sigma(D)-dependent. No sigma(M)-dependent promoter was identified. The typical housekeeping promoter P2sigA (sigma factor sigma(A)) was proven to be sigma(A)-dependent but also recognized by sigma(B). Similarly, the promoter of fba (fructose-1,6-bisphosphate aldolase) was confirmed to be sigma(B)-dependent but also functional with sigma(A). The study provided demonstrations of the broad applicability of the developed methods and produced original data on the analyzed promoters.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA17-06991S" target="_blank" >GA17-06991S: Construction of a model of regulatory network controlled by sigma factors of RNA polymerase in Corynebacterium glutamicum</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

AMB Express

ISSN

2191-0855

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

JUN 23

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

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UT code for WoS article

000404051700002

EID of the result in the Scopus database

2-s2.0-85021293503