Construction of in vitro transcription system for Corynebacterium glutamicum and its use in the recognition of promoters of different classes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00383121" target="_blank" >RIV/61388971:_____/12:00383121 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00253-012-4336-1" target="_blank" >http://dx.doi.org/10.1007/s00253-012-4336-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-012-4336-1" target="_blank" >10.1007/s00253-012-4336-1</a>

Result language

angličtina

Original language name

Construction of in vitro transcription system for Corynebacterium glutamicum and its use in the recognition of promoters of different classes

Original language description

To facilitate transcription studies in Corynebacterium glutamicum, we have developed an in vitro transcription system for this bacterium used as an industrial producer of amino acids and a model organism for actinobacteria. This system consists of C. glutamicum RNA polymerase (RNAP) core (alpha 2, beta, beta'), a sigma factor and a promoter-carrying DNA template, that is specifically recognized by the RNAP holoenzyme formed. The RNAP core was purified from the C. glutamicum strain with the modified rpoCgene, which produced His-tagged beta' subunit. The C. glutamicum sigA and sigH genes were cloned and overexpressed using the Escherichia coli plasmid vector, and the sigma subunits sigma(A) and sigma(H) were purified by affinity chromatography. Using the reconstituted C. glutamicum holo-RNAPs, recognition of the sigma(A)- and sigma(H)-dependent promoters and synthesis of the specific transcripts was demonstrated. The developed in vitro transcription system is a novel tool that can be us

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

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Volume of the periodical

96

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

521-529

UT code for WoS article

000308953100022

EID of the result in the Scopus database

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