Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σD and σH deciphered using computer modeling and point mutagenesis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00602636" target="_blank" >RIV/61388971:_____/25:00602636 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/25:10510830
Result on the web
<a href="https://link.springer.com/article/10.1007/s10822-024-00577-x" target="_blank" >https://link.springer.com/article/10.1007/s10822-024-00577-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10822-024-00577-x" target="_blank" >10.1007/s10822-024-00577-x</a>
Alternative languages
Result language
angličtina
Original language name
Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σD and σH deciphered using computer modeling and point mutagenesis
Original language description
This study aimed to reveal interactions of the stress response sigma subunits (factors) sigma D and sigma H of RNA polymerase and promoters in Gram-positive bacterium Corynebacterium glutamicum by combining wet-lab obtained data and in silico modeling. Computer modeling-guided point mutagenesis of C. glutamicum sigma H subunit led to the creation of a panel of sigma H variants. Their ability to initiate transcription from naturally occurring hybrid sigma D/sigma H-dependent promoter Pcg0441 and two control canonical promoters (sigma D-dependent PrsdA and sigma H-dependent PuvrD3) was measured and interpreted using molecular dynamics simulations of homology models of all complexes. The results led us to design the artificial hybrid promoter PD35H10 combining the10 element of the PuvrD3 promoter and the35 element of the PrsdA promoter. This artificial hybrid promoter PD35-rsdAH10-uvrD3 showed almost optimal properties needed for the bio-orthogonal transcription (not interfering with the native biological processes).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Computer-Aided Molecular Design
ISSN
0920-654X
e-ISSN
1573-4951
Volume of the periodical
39
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
16
Pages from-to
1
UT code for WoS article
001362550200001
EID of the result in the Scopus database
2-s2.0-85210099230