Protective effect of isoquercitrin against acute dextran sulfate sodium-induced rat colitis depends on the severity of tissue damage
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00467886" target="_blank" >RIV/61388971:_____/16:00467886 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/16:33159486
Result on the web
<a href="http://dx.doi.org/10.1016/j.pharep.2016.07.007" target="_blank" >http://dx.doi.org/10.1016/j.pharep.2016.07.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pharep.2016.07.007" target="_blank" >10.1016/j.pharep.2016.07.007</a>
Alternative languages
Result language
angličtina
Original language name
Protective effect of isoquercitrin against acute dextran sulfate sodium-induced rat colitis depends on the severity of tissue damage
Original language description
Background: Isoquercitrin (quercetin-3-O-beta-D-glucopyranoside) is a flavonoid that exhibited antioxidant and anti-inflammatory activities in a number of in vitro and in vivo studies. Experimental evidence from rodent models of inflammatory bowel disease is, however, lacking. This study was designed to examine whether isoquercitrin effectively and dose-dependently attenuates acute dextran sulfate sodium (DSS)-induced rat colitis. nMethods: Wistar rats were divided into negative control group (exposed to vehicle only), positive control group (DSS-induced colitis plus vehicle), low isoquercitrin group (DSS pretreated with isoquercitrin 1 mg/kg/day) and high isoquercitrin group (DSS with isoquercitrin 10 mg/kg/day). Isoquercitrin was administered daily for 14 days, and during the last 7 days rats drank DSS solution. The effect of isoquercitrin on DSS-induced colitis was assessed clinically (e.g. disease activity index), biochemically (tissue myeloperoxidase activity, local cyclooxygenase-2 expression), using histology (standard hematoxylin-eosin-based histomorphometry, immunohistochemical detection of inducible nitric oxide synthase) and hematology (blood count). nResults: Isoquercitrin dose-dependently ameliorated whole colon shortening and mitigated DSS-induced expression of cyclooxygenase-2 and inducible nitric oxide synthase in the descending segment of the organ. However, when different parts of colon were assessed histomorphometrically, the results did not globally support the protective role of this flavonoid. Tissue healing trends observable in the descending colon were not apparent in the rectum, where histological damage was most severe. nConclusions: We surmise that isoquercitrin may be effective in the prevention of acute colitis. Besides being dose-dependent, the potency of orally administered isoquercitrin may depend on the severity of tissue damage and/or on the site of its action.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP303%2F12%2FG163" target="_blank" >GBP303/12/G163: Centre of drug-dietary supplements interactions and nutrigenetics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Pharmacological Reports
ISSN
1734-1140
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
6
Country of publishing house
PL - POLAND
Number of pages
8
Pages from-to
1197-1204
UT code for WoS article
000388432100014
EID of the result in the Scopus database
2-s2.0-84988328456