Protein Extraction and Precipitation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00467977" target="_blank" >RIV/61388971:_____/16:00467977 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/B978-0-444-63688-1.00004-5" target="_blank" >http://dx.doi.org/10.1016/B978-0-444-63688-1.00004-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-444-63688-1.00004-5" target="_blank" >10.1016/B978-0-444-63688-1.00004-5</a>

Result language

angličtina

Original language name

Protein Extraction and Precipitation

Original language description

Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics.

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/LO1509" target="_blank" >LO1509: Prague infrastructure for structural biology and metabolomics II</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Proteomic Profiling and Analytical Chemistry: The Crossroads

ISBN

978-044463688-1

Number of pages of the result

11

Pages from-to

52-62

Number of pages of the book

282

Publisher name

Elsevier

Place of publication

Amsterdam

UT code for WoS chapter

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