Cofactor induced dissociation of the multifunctional multisubunit EcoR124I investigated using electromobility shift assays, AFM and SPR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00477805" target="_blank" >RIV/61388971:_____/17:00477805 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/c7ra07505g" target="_blank" >http://dx.doi.org/10.1039/c7ra07505g</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c7ra07505g" target="_blank" >10.1039/c7ra07505g</a>

Result language
angličtina
Original language name
Cofactor induced dissociation of the multifunctional multisubunit EcoR124I investigated using electromobility shift assays, AFM and SPR
Original language description
We have applied three techniques to the study of subunit assembly of the Type IC Restriction-Modification enzyme EcoR124I. This fully functional enzyme EcoR124I consists of a complex of the three subunits HsdR, HsdM and HsdS in a R2M2S1 stoichiometry, but is known to dissociate readily, releasing free HsdR and producing first an R-1-complex and then the core, DNA-binding methyltransferase (M2S1) complex. Analysis of the assembly pathway of this enzyme has previously employed gel retardation and Surface Plasmon Resonance (SPR), but the studies to date have not included the cofactors required for full enzyme activity. In this paper, we have also used atomic force microscopy (AFM)-based molecular volume measurements, and have analysed the effect of the cofactors ATP and AdoMet on enzyme stability and subunit assembly. We compare the data obtained from all three techniques and we show that they all give consistent results, but inherent differences in the methodologies provide additional information useful for the study of subunit assembly.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology

Project
<a href="/en/project/GA204%2F07%2F0325" target="_blank" >GA204/07/0325: Identification of amino acid residues of the HsdS and HsdR subunits required for correct assembly of Type I restriction-modification enzyme EcoR124I</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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