Protein composition of the phase I Coxiella burnetii soluble antigen prepared by extraction with trichloroacetic acid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00478402" target="_blank" >RIV/61388971:_____/17:00478402 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4149/av_2017_317" target="_blank" >http://dx.doi.org/10.4149/av_2017_317</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/av_2017_317" target="_blank" >10.4149/av_2017_317</a>

Result language

angličtina

Original language name

Protein composition of the phase I Coxiella burnetii soluble antigen prepared by extraction with trichloroacetic acid

Original language description

Q fever is a highly infectious, widespread airborne zoonosis caused by Coxiella burnetii bacterium. Humans usually acquire the disease by inhalation of contaminated aerosol produced by infected livestock. Vaccination is the most practical way for prevention and control of the disease in the exposed population. In this work, we reviewed the most important Q-fever outbreaks in Slovakia as well as the progress in vaccine development. One of them represents a soluble antigen complex produced by extraction with trichloroacetic acid from a highly purified C. burnetii phase I strain Nine Mile. It was developed at the Institute of Virology in Bratislava. The protein content of this vaccine was separated by gel electrophoresis and analyzed by mass spectrometry. The study has resulted in the identification of 39 bacterial proteins from which 12 were recognized as immunoreactive. Most of the proteins were involved in bacterium pathogenicity (41.6%) and cell wall maintenance (25%). Four of the immunoreactive proteins may possess the moonlighting activity. Definition of the vaccine components represents a prerequisite for vaccine standardization and approval by governmental authorities.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta virologica

ISSN

0001-723X

e-ISSN

—

Volume of the periodical

61

Issue of the periodical within the volume

3

Country of publishing house

SK - SLOVAKIA

Number of pages

8

Pages from-to

361-368

UT code for WoS article

000411298700018

EID of the result in the Scopus database

2-s2.0-85028620925