Reliable tool for detection of novel Coxiella burnetii antigens, using immobilized human polyclonal antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00474096" target="_blank" >RIV/61388971:_____/17:00474096 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jchromb.2016.08.044" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2016.08.044</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2016.08.044" target="_blank" >10.1016/j.jchromb.2016.08.044</a>

Result language

angličtina

Original language name

Reliable tool for detection of novel Coxiella burnetii antigens, using immobilized human polyclonal antibodies

Original language description

Coxiella burnetii (C. burnetii) is the etiological agent of a Q fever the re-emerging disease with considerable economic impact. Due to many similar symptoms with commonly occurring infections, its clinical diagnosis is very difficult. Thus, a strong effort should be taken to raise the awareness and develop a robust strategy for an accurate diagnosis. The identification of specific C. burnetii biomarkers could be valuable for a sensitive and selective diagnosis of the disease. Herein, we described a workflow to identify immunoreactive proteins of C. burnetii with a high confidence. It is based on immunocapturing of bacterial antigens by biofunctionalized magnetic microspheres, followed by tandem mass spectrometry (MS/MS) identification. We detected dozens of previously reported antigens and proposed 15 novel biomarkers, which specificity was confirmed by in silico epitope analysis. Among them, the cardiolipin synthetase participating in the synthesis of cardiolipin was recognized. This biomarker could play a critical role in the early management of acute Q fever and prevention of Q fever endocarditis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

—

Volume of the periodical

1047

Issue of the periodical within the volume

MAR 15 SI

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

84-91

UT code for WoS article

000397686200009

EID of the result in the Scopus database

2-s2.0-84994888576