Metabolic differentiation of surface and invasive cells of yeast colony biofilms revealed by gene expression profiling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00481684" target="_blank" >RIV/61388971:_____/17:00481684 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10363896

Result on the web

<a href="http://dx.doi.org/10.1186/s12864-017-4214-4" target="_blank" >http://dx.doi.org/10.1186/s12864-017-4214-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-017-4214-4" target="_blank" >10.1186/s12864-017-4214-4</a>

Result language

angličtina

Original language name

Metabolic differentiation of surface and invasive cells of yeast colony biofilms revealed by gene expression profiling

Original language description

Results: RNA sequencing revealed expression differences in 1245 genes with high statistical significance, indicating large genetically regulated metabolic differences between surface and invasive cells. Functional annotation analyses implicated genes involved in stress defense, peroxisomal fatty acid beta-oxidation, autophagy, protein degradation, storage compound metabolism and meiosis as being important in surface cells. In contrast, numerous genes with functions in nutrient transport and diverse synthetic metabolic reactions, including genes involved in ribosome biogenesis, biosynthesis and translation, were found to be important in invasive cells. Variation in gene expression correlated significantly with cell-type specific processes such as autophagy and storage compound accumulation as identified by microscopic and biochemical analyses. Expression profiling also provided indications of cell-specific regulations. Subsequent knockout strain analyses identified Gip2p, a regulatory subunit of type 1 protein phosphatase Glc7p, to be essential for glycogen accumulation in surface cells.nConclusions: This is the first study reporting genome wide differences between surface and invasive cells of yeast colony biofilms. New findings show that surface and invasive cells display very different physiology, adapting to different conditions in different colony areas and contributing to development and survival of the colony biofilm as a whole. Notably, surface and invasive cells of colony biofilms differ significantly from upper and lower cells ofnsmooth colonies adapted to plentiful laboratory conditions.n

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Genomics

ISSN

1471-2164

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

OCT 23

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

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UT code for WoS article

000413467900008

EID of the result in the Scopus database

2-s2.0-85031928357