Impact of Chemical Cross-Linking on Protein Structure and Function
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00489608" target="_blank" >RIV/61388971:_____/18:00489608 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/18:10374661
Result on the web
<a href="http://dx.doi.org/10.1021/acs.analchem.7b02863" target="_blank" >http://dx.doi.org/10.1021/acs.analchem.7b02863</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.7b02863" target="_blank" >10.1021/acs.analchem.7b02863</a>
Alternative languages
Result language
angličtina
Original language name
Impact of Chemical Cross-Linking on Protein Structure and Function
Original language description
Chemical cross-linking coupled with mass spectrometry is a popular technique for deriving structural information on proteins and protein complexes. Also, cross-linking has become a powerful tool for stabilizing macromolecular complexes for single-particle cryo-electronmicroscopy. However, an effect of cross-linking on protein structure and function should not be forgotten, and surprisingly, it has not been investigated in detail so far. Here, we used kinetic studies, mass spectrometry, and NMR spectroscopy to systematically investigate an impact of cross-linking on structure and function of human carbonic anhydrase and alcohol dehydrogenase 1 from Saccharomyces cerevisiae. We found that cross-linking induces rather local structural disturbances and the overall fold is preserved even at a higher cross-linker concentration. The results establish general experimental conditions for chemical cross-linking with minimal effect on protein structure and function.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
—
Volume of the periodical
90
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1104-1113
UT code for WoS article
000423011600012
EID of the result in the Scopus database
2-s2.0-85040673769