Communication between N terminus and loop2 tunes Orai activation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00489833" target="_blank" >RIV/61388971:_____/18:00489833 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.saa.2017.12.021" target="_blank" >http://dx.doi.org/10.1016/j.saa.2017.12.021</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.saa.2017.12.021" target="_blank" >10.1016/j.saa.2017.12.021</a>

Result language
angličtina
Original language name
Communication between N terminus and loop2 tunes Orai activation
Original language description
The CRAC channel gating involves the binding of STIM1 C-terminus to cytosolic N- and C-termini of Orai. The C-termini of Orai is the main binding partner however N-termini also have been found necessary for Orai channel function. Despite a highly conserved ETON region Orai1 and Orai3 differ in length of the ETON region. Our aim here was to study the reasons for different behavior of these two isoforms and to find the differences in structural requirement for gating and function. While there is no interaction between the N-terminus and loop2 in Orai3, we predict that interactions between loop2 and Orai1 N-terminus results into masking the STIM1 coupling sites which are located either at N-terminus or loop2 or both, thus loop2 either directly or allosterically affects the STIM1-Orai coupling in an isoform specific manner. Thus along with the requirement of conserved ETON region a fine tuning between N-termini and loop2 conformations has an essential role in maintaining permissive conformation of channel and store-operated function of Orai channel.nn
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
<a href="/en/project/LTC17069" target="_blank" >LTC17069: Understanding different aspects of structure and function of cation translocation systems.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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