Novel assay for the toxicity evaluation of nanoscale zero-valent iron and derived nanomaterials based on lipid peroxidation in bacterial species

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00498769" target="_blank" >RIV/61388971:_____/18:00498769 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10386220 RIV/61989592:15310/18:73588958

Result on the web

<a href="http://dx.doi.org/10.1016/j.chemosphere.2018.09.029" target="_blank" >http://dx.doi.org/10.1016/j.chemosphere.2018.09.029</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2018.09.029" target="_blank" >10.1016/j.chemosphere.2018.09.029</a>

Result language

angličtina

Original language name

Novel assay for the toxicity evaluation of nanoscale zero-valent iron and derived nanomaterials based on lipid peroxidation in bacterial species

Original language description

Nano-scale zero-valent iron (nZVI) began attracting research attention in remediation practice in recent decades as a prospective nanomaterial applicable to various contaminated matrices. Despite concerns about the negative effects of nanomaterials on ecosystems, the number of reliable toxicity tests is limited. We have developed a test based on the evaluation of oxidative stress (OS). The test employed the analysis of a typical OS marker (malondialdehyde, MDA), after exposure of six bacterial strains to the tested nanomaterial. We also attempted to use other OS and cell membrane damage assays, including the determination of glutathione and lactate dehydrogenase, respectively. However, we found that the components of these assays interfered with nZVI, therefore, these tests were not applicable. The MDA assay was tested using nZVI and three newly engineered oxide shell nZVI materials with different oxide thicknesses. Six different bacterial species were employed, and the results showed that the test was fully applicable for the concentrations of nanomaterials used in remediation practice (0.1-10 g/L). MDA was produced in a dose-response manner, and the bacteria showed a similar response toward pure pyrophoric nZVI, reaching EC50 values of 0.3-1.1 g/L. We observed different responses in the absolute production of MDA, however, the MDA concentrations were correlated with the cell membrane surfaces of the individual strains (R > 0.75, P < 0.09). Additionally, the EC50 values correlated with the thickness of the oxide shells (except for Escherichia coli: R > 0.95, P < 0.05), documenting the reliability of the assay, where reactivity was confirmed to be an important factor for reactive oxygen species production.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

<a href="/en/project/TE01020218" target="_blank" >TE01020218: Environmental friendly nanotechnologies and biotechnologies in water and soil treatment</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemosphere

ISSN

0045-6535

e-ISSN

—

Volume of the periodical

213

Issue of the periodical within the volume

DEC

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

568-577

UT code for WoS article

000448493700061

EID of the result in the Scopus database

2-s2.0-85053827195