Oxidative stress in microbes after exposure to iron nanoparticles: analysis of aldehydes as oxidative damage products of lipids and proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00519320" target="_blank" >RIV/61388971:_____/19:00519320 - isvavai.cz</a>

Alternative codes found

RIV/67985874:_____/19:00519320 RIV/00216208:11310/19:10403145 RIV/61989592:15310/19:73600637

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs11356-019-06370-w" target="_blank" >https://link.springer.com/article/10.1007%2Fs11356-019-06370-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11356-019-06370-w" target="_blank" >10.1007/s11356-019-06370-w</a>

Result language

angličtina

Original language name

Oxidative stress in microbes after exposure to iron nanoparticles: analysis of aldehydes as oxidative damage products of lipids and proteins

Original language description

Due to their enhanced reactivity, metal and metal-oxide nanoscale zero-valent iron (nZVI) nanomaterials have been introduced into remediation practice. To ensure that environmental applications of nanomaterials are safe, their possible toxic effects should be described. However, there is still a lack of suitable toxicity tests that address the specific mode of action of nanoparticles, especially for nZVI. This contribution presents a novel approach for monitoring one of the most discussed adverse effects of nanoparticles, i.e., oxidative stress (OS). We optimized and developed an assay based on headspace-SPME-GC-MS analysis that enables the direct determination of volatile oxidative damage products (aldehydes) of lipids and proteins in microbial cultures after exposure to commercial types of nZVI. The method employs PDMS/DVB SPME fibers and pentafluorobenzyl derivatization, and the protocol was successfully tested using representatives of bacteria, fungi, and algae. Six aldehydes, namely, formaldehyde, acrolein, methional, benzaldehyde, glyoxal, and methylglyoxal, were detected in the cultures, and all of them exhibited dose-dependent sigmoidal responses. The presence of methional, which was detected in all cultures except those including an algal strain, documents that nZVI also caused oxidative damage to proteins in addition to lipids. The most sensitive toward nZVI exposure in terms of aldehyde production was the yeast strain Saccharomyces cerevisiae, which had an EC50 value of 0.08 g/L nZVI. To the best of our knowledge, this paper is the first to document the production of aldehydes resulting from lipids and proteins as a result of OS in microorganisms from different kingdoms after exposure to iron nanoparticles.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/TE01020218" target="_blank" >TE01020218: Environmental friendly nanotechnologies and biotechnologies in water and soil treatment</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental Science and Pollution Research

ISSN

0944-1344

e-ISSN

—

Volume of the periodical

26

Issue of the periodical within the volume

32 SI

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

33670-33682

UT code for WoS article

000501757400087

EID of the result in the Scopus database

2-s2.0-85074048363