Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00499508" target="_blank" >RIV/61388971:_____/18:00499508 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/18:00499508

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals

Original language description

Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest. nHere we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the wound, and effective release of antisense RNA in the wound. Embedding of nanodiamond-siRNA systems into nanofiber scaffold enables continuous release of siRNA and maintaining the stable siRNA concentration in the wound site resulting in a promotion of wound healing. nWe developed FND-siRNA complexes specific to MMP-9 that efficiently inhibit the expression of target MMP-9 mRNA. The complexes were embedded into core/shell nanofibers from PVA and PCL, visualized by confocal microscopy, and characterized by electron microscopy. Real-time PCR was used to assess the silencing effect of siRNA that has been delivered to target murine fibroblasts by FND released from nanofiber dressing. Nanofiber system with embedded FNDs was applied on wounds in diabetic animal models to evaluate its suitability regarding short and long term toxicity, efficacy, and handling in vivo. n

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NV15-33094A" target="_blank" >NV15-33094A: Nanofiber drug carriers for controlled release of wound healing substances based on the encapsulation of functionalized nanodiamond particles</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

CONFERENCE PROCEEDINGS NANOCON 2017

ISBN

9788087294819

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

467-472

Publisher name

Tnager Ltd.

Place of publication

Brno

Event location

Brno

Event date

Oct 18, 2017

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000452823300076