Topical siRNA therapy of diabetic-like wound healing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00616861" target="_blank" >RIV/61388971:_____/25:00616861 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/25:00616861 RIV/61388963:_____/25:00616861 RIV/46747885:24530/25:00013614 RIV/00216208:11310/25:10498935

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2025/tb/d4tb01547a" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2025/tb/d4tb01547a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d4tb01547a" target="_blank" >10.1039/d4tb01547a</a>

Result language

angličtina

Original language name

Topical siRNA therapy of diabetic-like wound healing

Original language description

Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of in vivo toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Materials Chemistry B

ISSN

2050-750X

e-ISSN

2050-7518

Volume of the periodical

13

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

1037-1051

UT code for WoS article

001369613300001

EID of the result in the Scopus database

2-s2.0-85211064871