Characterization of the catabolic pathway of diclofenac in Raoultella sp. KDF8

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00504027" target="_blank" >RIV/61388971:_____/19:00504027 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0964830518310631?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0964830518310631?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ibiod.2018.11.013" target="_blank" >10.1016/j.ibiod.2018.11.013</a>

Result language
angličtina
Original language name
Characterization of the catabolic pathway of diclofenac in Raoultella sp. KDF8
Original language description
The removal of diclofenac, which is a widely used non-steroidal anti-inflammatory drug and a dangerous environmental contaminant of surface water, was studied. Its complete degradation by Raoultella sp. KDF8 to primary metabolic products was described with individual metabolites identified by mass spectrometry. The Raoultella strain KDF8 was cultivated with unlabelled and (13)C6-labelled diclofenac. A total of 32 compounds were detected as intermediates in diclofenac metabolism by high resolution tandem mass spectrometry. Furthermore, proteomic analysis showed that the presence of diclofenac resulted in the upregulation of 24 enzymes associated with the benzoate, catechol, protocatechuate and ketoadipate pathways. The presence of genes encoding specific enzymes, such as catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and quercetin 2,3-dioxygenase, has been verified by sequence analysis of PCR products. The description of diclofenac metabolism by strain KDF8 provides a detailed view of the degradation of xenobiotic aromatic compounds by microorganisms in general.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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