Alternative Biosynthetic Starter Units Enhance the Structural Diversity of Cyanobacterial Lipopeptides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00506161" target="_blank" >RIV/61388971:_____/19:00506161 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/19:00505637 RIV/60076658:12310/19:43899167
Result on the web
<a href="https://aem.asm.org/content/85/4/e02675-18" target="_blank" >https://aem.asm.org/content/85/4/e02675-18</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/AEM.02675-18" target="_blank" >10.1128/AEM.02675-18</a>
Alternative languages
Result language
angličtina
Original language name
Alternative Biosynthetic Starter Units Enhance the Structural Diversity of Cyanobacterial Lipopeptides
Original language description
Puwainaphycins (PUWs) and minutissamides (MINs) are structurally analogous cyclic lipopeptides possessing cytotoxic activity. Both types of compound exhibit high structural variability, particularly in the fatty acid (FA) moiety. Although a biosynthetic gene cluster responsible for synthesis of several PUW variants has been proposed in a cyanobacterial strain, the genetic background for MINs remains unexplored. Herein, we report PUW/MIN biosynthetic gene clusters and structural variants from six cyanobacterial strains. Comparison of biosynthetic gene clusters indicates a common origin of the PUW/MIN hybrid nonribosomal peptide synthetase and polyketide synthase. Surprisingly, the biosynthetic gene clusters encode two alternative biosynthetic starter modules, and analysis of structural variants suggests that initiation by each of the starter modules results in lipopeptides of differing lengths and FA substitutions. Among additional modifications of the FA chain, chlorination of minutissamide D was explained by the presence of a putative halogenase gene in the PUW/MIN gene cluster of Anabaena minutissima strain UTEX B 1613. We detected PUW variants bearing an acetyl substitution in Symplocastrum muelleri strain NIVA-CYA 644, consistent with an O-acetyltransferase gene in its biosynthetic gene cluster. The major lipopeptide variants did not exhibit any significant antibacterial activity, and only the PUW F variant was moderately active against yeast, consistent with previously published data suggesting that PUWs/MINs interact preferentially with eukaryotic plasma membranes. n
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied and Environmental Microbiology
ISSN
0099-2240
e-ISSN
—
Volume of the periodical
85
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
e02675-18
UT code for WoS article
000457934800020
EID of the result in the Scopus database
2-s2.0-85061130220