Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00537796" target="_blank" >RIV/61388971:_____/20:00537796 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/20:00537796 RIV/67985939:_____/20:00537796 RIV/00216224:14310/20:00114330
Result on the web
<a href="https://doi.org/10.1016/j.hal.2020.101849" target="_blank" >https://doi.org/10.1016/j.hal.2020.101849</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.hal.2020.101849" target="_blank" >10.1016/j.hal.2020.101849</a>
Alternative languages
Result language
angličtina
Original language name
Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
Original language description
Puwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10617 - Marine biology, freshwater biology, limnology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Harmful Algae
ISSN
1568-9883
e-ISSN
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Volume of the periodical
96
Issue of the periodical within the volume
Jun 2020
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
101849
UT code for WoS article
000541912700014
EID of the result in the Scopus database
2-s2.0-85086601564