Rapid screening of very long-chain fatty acids from microorganisms

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00518107" target="_blank" >RIV/61388971:_____/19:00518107 - isvavai.cz</a>
Alternative codes found
RIV/67985939:_____/19:00518107 RIV/00216208:11310/19:10409537 RIV/60461373:22330/19:43918587
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2019.460365" target="_blank" >10.1016/j.chroma.2019.460365</a>

Result language
angličtina
Original language name
Rapid screening of very long-chain fatty acids from microorganisms
Original language description
The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:19, 1 omega 9, 24:6 omega 3, 26:0, 26:1 omega 9, 28:0, 28:1 omega 9, 28:2 omega 6 and 28:8 omega 3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (R1COO- and R2COO-). The limit of detection was determined at 10 nmol/L (similar to 11 pg/mu L) in triacylglycerols and 8.6 nmoles/L (similar to 8 pg/mu L) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology

Project
<a href="/en/project/GA17-00027S" target="_blank" >GA17-00027S: Very long chain fatty acids from microorganisms</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)