Cytotoxicity of the effector protein BteA was attenuated in Bordetella pertussis by insertion of an alanine residue
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00533321" target="_blank" >RIV/61388971:_____/20:00533321 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/20:00533321
Result on the web
<a href="https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008512" target="_blank" >https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008512</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.ppat.1008512" target="_blank" >10.1371/journal.ppat.1008512</a>
Alternative languages
Result language
angličtina
Original language name
Cytotoxicity of the effector protein BteA was attenuated in Bordetella pertussis by insertion of an alanine residue
Original language description
Bordetella bronchisepticaandBordetella pertussisare closely related respiratory pathogens that evolved from a common bacterial ancestor. WhileB.bronchisepticahas an environmental reservoir and mostly establishes chronic infections in a broad range of mammals,B.pertussisis a human-specific pathogen causing acute pulmonary pertussis in infants and whooping cough illness in older humans. Both species employ a type III secretion system (T3SS) to inject a cytotoxic BteA effector protein into host cells. However, compared to the high BteA-mediated cytotoxicity ofB.bronchiseptica, the cytotoxicity induced byB.pertussisBteA (BpBteA) appears to be quite low and this has been attributed to the reduced T3SS gene expression inB.pertussis. We show that the presence of an alanine residue inserted at position 503 (A503) ofBpBteA accounts for its strongly attenuated cytotoxic potency. The deletion of A503 fromBpBteA greatly enhanced the cytotoxic activity ofB.pertussisB1917 on mammalian HeLa cells and expression ofBpBteA Delta A503 was highly toxic toSaccharomyces cerevisiaecells.Vice versa, insertion of A503 intoB.bronchisepticaBteA (BbBteA) strongly decreased its cytotoxicity to yeast and HeLa cells. Moreover, the production ofBpBteA Delta A503 increased virulence ofB.pertussisB1917 in the mouse model of intranasal infection (reduced LD50) but yielded less inflammatory pathology in infected mouse lungs at sublethal infectious doses. This suggests that A503 insertion in the T3SS effectorBpBteA may represent an evolutionary adaptation that fine-tunesB.pertussisvirulence and host immune response.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS Pathogens
ISSN
1553-7366
e-ISSN
—
Volume of the periodical
16
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
e1008512
UT code for WoS article
000561392400003
EID of the result in the Scopus database
2-s2.0-85089787575