Natural Killer Cell Activation Receptor NKp30 Oligomerization Depends on ItsN-Glycosylation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00533400" target="_blank" >RIV/61388971:_____/20:00533400 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/20:00533400 RIV/00216208:11310/20:10413388
Result on the web
<a href="https://www.mdpi.com/2072-6694/12/7/1998" target="_blank" >https://www.mdpi.com/2072-6694/12/7/1998</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cancers12071998" target="_blank" >10.3390/cancers12071998</a>
Alternative languages
Result language
angličtina
Original language name
Natural Killer Cell Activation Receptor NKp30 Oligomerization Depends on ItsN-Glycosylation
Original language description
NKp30 is one of the main human natural killer (NK) cell activating receptors used in directed immunotherapy. The oligomerization of the NKp30 ligand binding domain depends on the length of the C-terminal stalk region, but our structural knowledge of NKp30 oligomerization and its role in signal transduction remains limited. Moreover, ligand binding of NKp30 is affected by the presence and type ofN-glycosylation. In this study, we assessed whether NKp30 oligomerization depends on itsN-glycosylation. Our results show that NKp30 forms oligomers when expressed in HEK293S GnTI(-)cell lines with simpleN-glycans. However, NKp30 was detected only as monomers after enzymatic deglycosylation. Furthermore, we characterized the interaction between NKp30 and its best-studied cognate ligand, B7-H6, with respect to glycosylation and oligomerization, and we solved the crystal structure of this complex with glycosylated NKp30, revealing a new glycosylation-induced mode of NKp30 dimerization. Overall, this study provides new insights into the structural basis of NKp30 oligomerization and explains how the stalk region and glycosylation of NKp30 affect its ligand affinity. This furthers our understanding of the molecular mechanisms involved in NK cell activation, which is crucial for the successful design of novel NK cell-based targeted immunotherapeutics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cancers (Basel)
ISSN
2072-6694
e-ISSN
—
Volume of the periodical
12
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
24
Pages from-to
1998
UT code for WoS article
000554160800001
EID of the result in the Scopus database
2-s2.0-85088272571