Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00535755" target="_blank" >RIV/61388971:_____/20:00535755 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/20:43920674
Result on the web
<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229430" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229430</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0229430" target="_blank" >10.1371/journal.pone.0229430</a>
Alternative languages
Result language
angličtina
Original language name
Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach
Original language description
Many compounds have the potential to harm pancreatic beta-cells, organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p'-DDT exposure among proteins expressed in NES2Y human pancreatic beta-cells employing 2-D electrophoresis. We exposed NES2Y cells to a high concentration (150 mu M, LC96 after 72 hours) of p,p'-DDT for 24 and 30 hours and determined proteins with changed expression using 2-D electrophoresis. We have found 22 proteins that changed their expression. They included proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins involved in the maintenance of the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). The proteins we have identified may serve as indicators of p,p'-DDT toxicity in beta-cells in future studies, including long-term exposure to environmentally relevant concentrations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
—
Volume of the periodical
15
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
e0229430
UT code for WoS article
000588370000016
EID of the result in the Scopus database
2-s2.0-85094863054