Bringing SEM and MSI closer than ever before: Visualizing aspergillus and pseudomonas infection in the rat lungs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00536784" target="_blank" >RIV/61388971:_____/20:00536784 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/20:73602619 RIV/44555601:13440/20:43895624 RIV/00216208:11310/20:10421381
Result on the web
<a href="https://www.mdpi.com/2309-608X/6/4/257" target="_blank" >https://www.mdpi.com/2309-608X/6/4/257</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/jof6040257" target="_blank" >10.3390/jof6040257</a>
Alternative languages
Result language
angličtina
Original language name
Bringing SEM and MSI closer than ever before: Visualizing aspergillus and pseudomonas infection in the rat lungs
Original language description
A procedure for processing frozen rat lung tissue sections for scanning electron microscopy (SEM) from deeply frozen samples initially collected and stored for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed. The procedure employed slow thawing of the frozen sections while floating on the surface and melting in a fixative solution. After the float-washing step, the sections were dehydrated in a graded ethanol series and dried in a critical point dryer. The SEM generated images with well-preserved structures, allowing for monitoring of bacterial cells and fungal hyphae in the infected tissue. Importantly, the consecutive nonfixed frozen sections were fully compatible with MALDI-MSI, providing molecular biomarker maps of Pseudomonas aeruginosa. The protocol enables bimodal image fusion in the in-house software CycloBranch, as demonstrated by SEM and MALDI-MSI.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Fungi
ISSN
2309-608X
e-ISSN
—
Volume of the periodical
6
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
257
UT code for WoS article
000601657200001
EID of the result in the Scopus database
2-s2.0-85094863357