Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00537734" target="_blank" >RIV/61388971:_____/20:00537734 - isvavai.cz</a>
Result on the web
<a href="https://bio-protocol.org/e3528?sid=f85bba85-5c8b-4cd0-96d2-e2c9090f9d97&sousouid=20210118012731766&ptype=0&px=1&intwzid=3528" target="_blank" >https://bio-protocol.org/e3528?sid=f85bba85-5c8b-4cd0-96d2-e2c9090f9d97&sousouid=20210118012731766&ptype=0&px=1&intwzid=3528</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.21769/BioProtoc.3528" target="_blank" >10.21769/BioProtoc.3528</a>
Alternative languages
Result language
angličtina
Original language name
Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
Original language description
Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a bottleneck in the elucidation of its genomic function. Antisense RNA (asRNA) mediated approach renders the study of essential genes possible by suppressing (but not completely eliminating) expression of the target gene, thus allowing them to function to some extent. Recently, we have successfully implemented this approach using the strong endogenous promoter of the psbAl gene (D1 subunit of Photosystem II) introduced into a highcopy replicative plasmid (pAM1956) to suppress the transcript level of the target gene alr0277 (encoding a sigma factor, SigJ/Alr0277) in Anabaena. This protocol represents an efficient and easy procedure to further explore the functional genomics, expanding the scope of basic and applied research in these ecologically important cyanobacteria.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/LO1416" target="_blank" >LO1416: Algatech plus</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bio-protocol
ISSN
2331-8325
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
e3528
UT code for WoS article
000583091400009
EID of the result in the Scopus database
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