Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00537940" target="_blank" >RIV/61388971:_____/21:00537940 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-1126-5_11" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-1126-5_11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-1126-5_11" target="_blank" >10.1007/978-1-0716-1126-5_11</a>

Result language
angličtina
Original language name
Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry
Original language description
Protein hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) can be used to study interactions of proteins with various ligands, to describe the effects of mutations, or to reveal structural responses of proteins to different experimental conditions. It is often described as a method with virtually no limitations in terms of protein size or sample composition. While this is generally true, there are, however, ligands or buffer components that can significantly complicate the analysis. One such compound, that can make HDX-MS troublesome, is DNA. In this chapter, we will focus on the analysis of protein–DNA interactions, describe the detailed protocol, and point out ways to overcome the complications arising from the presence of DNA.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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