Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542218" target="_blank" >RIV/61388971:_____/21:00542218 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/21:00540701 RIV/00216208:11310/21:10439794
Result on the web
<a href="https://www.nature.com/articles/s42003-021-01694-1" target="_blank" >https://www.nature.com/articles/s42003-021-01694-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s42003-021-01694-1" target="_blank" >10.1038/s42003-021-01694-1</a>
Alternative languages
Result language
angličtina
Original language name
Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools
Original language description
Fluorescence-detected linear dichroism microscopy allows observing various molecular processes in living cells, as well as obtaining quantitative information on orientation of fluorescent molecules associated with cellular features. Such information can provide insights into protein structure, aid in development of genetically encoded probes, and allow determinations of lipid membrane properties. However, quantitating and interpreting linear dichroism in biological systems has been laborious and unreliable. Here we present a set of open source ImageJ-based software tools that allow fast and easy linear dichroism visualization and quantitation, as well as extraction of quantitative information on molecular orientations, even in living systems. The tools were tested on model synthetic lipid vesicles and applied to a variety of biological systems, including observations of conformational changes during G-protein signaling in living cells, using fluorescent proteins. Our results show that our tools and model systems are applicable to a wide range of molecules and polarization-resolved microscopy techniques, and represent a significant step towards making polarization microscopy a mainstream tool of biological imaging. Expanding on their previous work, Bondar et al present open source software tools to reliably quantify linear dichroism and determine molecular orientations. They demonstrate the utility of the tools by imaging synthetic lipid vesicles and as well as fluorescently labelled proteins in living cells
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Communications Biology
ISSN
2399-3642
e-ISSN
2399-3642
Volume of the periodical
4
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
189
UT code for WoS article
000620241900002
EID of the result in the Scopus database
2-s2.0-85100869737