SAP domain forms a flexible part of DNA aperture in Ku70/80
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542410" target="_blank" >RIV/61388971:_____/21:00542410 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/21:00124528
Result on the web
<a href="https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15732" target="_blank" >https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15732</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/febs.15732" target="_blank" >10.1111/febs.15732</a>
Alternative languages
Result language
angličtina
Original language name
SAP domain forms a flexible part of DNA aperture in Ku70/80
Original language description
Nonhomologous end joining (NHEJ) is a DNA repair mechanism that religates double-strand DNA breaks to maintain genomic integrity during the entire cell cycle. The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. Here, we describe intramolecular interactions of the Ku70 C-terminal domain, known as the SAP domain. Using single-particle cryo-electron microscopy, mass spectrometric analysis of intermolecular cross-linking and molecular modelling simulations, we captured variable positions of the SAP domain depending on DNA binding. The first position was localized at the DNA aperture in the Ku70/80 apo form but was not observed in the DNA-bound state. The second position, which was observed in both apo and DNA-bound states, was found below the DNA aperture, close to the helical arm of Ku70. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEBS Journal
ISSN
1742-464X
e-ISSN
1742-4658
Volume of the periodical
288
Issue of the periodical within the volume
14
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
4382-4393
UT code for WoS article
000618236000001
EID of the result in the Scopus database
2-s2.0-85101481378