Arabidopsis DNA polymerase lambda mutant is mildly sensitive to DNA double strand breaks but defective in integration of a transgene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00447194" target="_blank" >RIV/61389030:_____/15:00447194 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3389/fpls.2015.00357" target="_blank" >http://dx.doi.org/10.3389/fpls.2015.00357</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2015.00357" target="_blank" >10.3389/fpls.2015.00357</a>

Result language

angličtina

Original language name

Arabidopsis DNA polymerase lambda mutant is mildly sensitive to DNA double strand breaks but defective in integration of a transgene

Original language description

The DNA double-strand break (DSB) is a critical type of damage, and can be induced by both endogenous sources (e.g., errors of oxidative metabolism, transposable elements, programmed meiotic breaks, or perturbation of the DNA replication fork) and exogenous sources (e.g., ionizing radiation or radiomimetic chemicals). Although higher plants, like mammals, are thought to preferentially repair DSBs via nonhomologous end joining (NHEJ), much remains unclear about plant DSB repair pathways. Our reverse genetic approach suggests that DNA polymerase lambda is involved in DSB repair in Arabidopsis. The Arabidopsis T-DNA insertion mutant (atpol lambda-1) displayed sensitivity to both gamma-irradiation and treatment with radiomimetic reagents, but not to otherDNA damaging treatments. The atpol lambda-1 mutant showed a moderate sensitivity to DSBs, while Arabidopsis Ku70 and DNA ligase 4 mutants (atku70-3 and atlig4-2), both of which play critical roles in NHEJ, exhibited a hypersensitivity to

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

MAY 27

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

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UT code for WoS article

000357009900001

EID of the result in the Scopus database

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