The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00543232" target="_blank" >RIV/61388971:_____/21:00543232 - isvavai.cz</a>

Alternative codes found

RIV/67985939:_____/21:00544601

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S1388198121000937?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1388198121000937?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbalip.2021.158965" target="_blank" >10.1016/j.bbalip.2021.158965</a>

Result language

angličtina

Original language name

The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote

Original language description

The structural challenges faced by eukaryotic cells through the cell cycle are key for understanding cell viability and proliferation. We tested the hypothesis that the biosynthesis of structural lipids is linked to the cell cycle. If true, this would suggest that the cell's structure is important for progress through and perhaps even control of the cell cycle. Lipidomics (P-31 NMR and MS), proteomics (Western immunoblotting) and transcriptomics (RT-qPCR) techniques were used to profile the lipid fraction and characterise aspects of its metabolism at seven stages of the cell cycle of the model eukaryote, Desmodesmus quadricauda. We found considerable, transient increases in the abundance of phosphatidylethanolamine during the G(1) phase (+35%, ethanolamine phosphate cytidylyltransferase increased 2.5x) and phosphatidylglycerol (+100%, phosphatidylglycerol synthase increased 22x) over the G1/pre-replication phase boundary. The relative abundance of phosphatidylcholine fell by similar to 35% during the G1. N-Methyl transferases for the conversion of phosphatidylethanolamine into phosphatidylcholine were not found in the de novo transcriptome profile, though a choline phosphate transferase was found, suggesting that the Kennedy pathway is the principal route for the synthesis of PC. The fatty acid profiles of the four most abundant lipids suggested that these lipids were not generally converted between one another. This study shows for the first time that there are considerable changes in the biosynthesis of the three most abundant phospholipid classes in the normal cell cycle of D. quadricauda, by margins large enough to elicit changes to the physical properties of membranes.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids

ISSN

1388-1981

e-ISSN

1879-2618

Volume of the periodical

1866

Issue of the periodical within the volume

8

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

158965

UT code for WoS article

000656122900009

EID of the result in the Scopus database

2-s2.0-85107319643