Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00545801" target="_blank" >RIV/61388971:_____/21:00545801 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/21:00545801 RIV/67985874:_____/21:00545801 RIV/00216208:11110/21:10430463 RIV/00216208:11310/21:10430463 RIV/00216224:14740/21:00124533

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2021/EN/D1EN00080B" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2021/EN/D1EN00080B</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d1en00080b" target="_blank" >10.1039/d1en00080b</a>

Result language

angličtina

Original language name

Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells

Original language description

Copper oxide nanoparticles (CuO NPs) are widely used in industry. Once released, they can enter the soil system and endanger organisms living in this environment. Therefore, monitoring the NP impact on soil organisms and identification of suitable biomarkers associated with NP pollution are required. In this study, immune effector cells of the earthworm Eisenia andrei, amoebocytes, were exposed to environmentally relevant sublethal concentrations of CuO NPs (1, 10, and 100 mu g mL(-1) of Cu) and their impact on the cellular and subcellular levels, as well as on the mRNA levels of molecules involved in the defense reactions, was assessed in vitro. CuO NPs decreased the viability of both amoebocyte subpopulations by 40% at the highest concentration tested (100 mu g mL(-1) of Cu). Further, CuO NPs caused significant attenuation of the phagocytic function of hyaline amoebocytes after 6 and 24 hours of exposure, by 37 and 25%, respectively. The concentration of the lipid peroxidation subproduct, malondialdehyde, was 10 times elevated in cells exposed to CuO NPs (100 mu g mL(-1) of Cu) after 6 hours of exposure. We hypothesize that malondialdehyde may induce DNA breaks, cell cycle arrest, and subsequent cell death. Electron microscopy showed the interaction between CuO NPs and immune effector cells, amoebocytes. Moreover, aggregates of CuO NPs were shown to be engulfed and located in the cytoplasm of these cells. However, data from all experiments indicate that the observed effects of CuO NPs on earthworm coelomocytes were caused mainly by the dissolved Cu2+ ions derived from nanoparticles (NPs). The determination of effective parameters such as oxidative stress, immune reactivity, and genotoxicity would provide valuable comprehension and data for environmental assessment of NP impact on soil organisms.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental Science-Nano

ISSN

2051-8153

e-ISSN

2051-8161

Volume of the periodical

8

Issue of the periodical within the volume

9

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

2464-2477

UT code for WoS article

000674973000001

EID of the result in the Scopus database

2-s2.0-85115241590