Separation of regioisomers and enantiomers of triacylglycerols containing branched fatty acids (iso and/or anteiso)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00546762" target="_blank" >RIV/61388971:_____/21:00546762 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/21:10437181
Result on the web
<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202000320" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202000320</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202000320" target="_blank" >10.1002/elps.202000320</a>
Alternative languages
Result language
angličtina
Original language name
Separation of regioisomers and enantiomers of triacylglycerols containing branched fatty acids (iso and/or anteiso)
Original language description
A combination of two chromatographic and one enzymatic methods was used for identification of the molecular species of triacylglycerols (TAGs) from Streptomyces avermitilis. Streptomyces avermitliswas cultured on various carbon sources and the ratio of iso- (i-FAs), anteiso- (ai-FAs), and straight-chain- (n-FAs) fatty acids was modified by precursor-directed biosynthesis. Saturated TAGs were separated from other lipids (including TAGs containing unsaturated FAs) using Ag+ ion cartridges. Analysis of TAGs wereperformed by RP-HPLC/ESI+ tandem mass spectrometry. Both the synthetically prepared sn-TAGs and the natural mixture of TAGmolecular species of wereseparated and identified by tandem MS. The structures of synthetic TAGs werefurther confirmed by pancreatic lipase, which cleaves sn-TAGs into sn-2-monoacylglycerols. The retention times (tR) of the individual regioisomers and enantiomers were found to be depend on the structure of the TAGs. If one branched acyl (iso or anteiso) is present in the TAG molecule, then the elution order is enantiomer (n/n/br), opposite enantiomer (br/n/n), regioisomer (n/br/n). In the case where two branched acyls are in the TAG molecule, the order of the elution is different, that is, br/n/br, n/br/br, br/br/n. In all cases, it was further demonstrated that tandem MS of either synthetically prepared TAGs or TAGs obtained from natural material, that is, n-16:0/ai-15:0/n-16:0 and i-16:0/n-15:0/i-16:0 are identical. Unfortunately, it is not possible to distinguish by ESI+ tandem MS such TAGs, which differ only in the branching of the acyls. The results of our analyses of TAGs are in good agreement with previously published data in other streptomycetes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
42
Issue of the periodical within the volume
17-18
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1832-1843
UT code for WoS article
000605174600001
EID of the result in the Scopus database
2-s2.0-85099064420