Secretory IgAN-glycans contribute to the protection againstE. coliO55 infection of germ-free piglets

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00550089" target="_blank" >RIV/61388971:_____/21:00550089 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/21:73602947
Result on the web
<a href="https://www.nature.com/articles/s41385-020-00345-8" target="_blank" >https://www.nature.com/articles/s41385-020-00345-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41385-020-00345-8" target="_blank" >10.1038/s41385-020-00345-8</a>

Result language
angličtina
Original language name
Secretory IgAN-glycans contribute to the protection againstE. coliO55 infection of germ-free piglets
Original language description
Mucosal surfaces are colonized by highly diverse commensal microbiota. Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens. Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans. In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo. Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removedN-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenicE. coliO55 challenge. SIgA, Fab, and Fc-SC were protective, whereas removal ofN-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival. In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination ofE. coliO55. These findings highlight the role of SIgA-associatedN-glycans in protection. Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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