Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00551051" target="_blank" >RIV/61388971:_____/21:00551051 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/21:00556422 RIV/61388963:_____/21:00551051 RIV/00216208:11310/21:10437999
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/jacs.1c07771" target="_blank" >https://pubs.acs.org/doi/10.1021/jacs.1c07771</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/jacs.1c07771" target="_blank" >10.1021/jacs.1c07771</a>

Result language
angličtina
Original language name
Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules
Original language description
Covalent labeling of proteins in combination with mass spectrometry has been established as a complementary technique to classical structural methods, such as X-ray, NMR, or cryogenic electron microscopy (Cryo-EM), used for protein structure determination. Although the current covalent labeling techniques enable the protein solvent accessible areas with sufficient spatial resolution to be monitored, there is still high demand for alternative, less complicated, and inexpensive approaches. Here, we introduce a new covalent labeling method based on fast fluoroalkylation of proteins (FFAP). FFAP uses fluoroalkyl radicals formed by reductive decomposition of Togni reagents with ascorbic acid to label proteins on a time scale of seconds. The feasibility of FFAP to effectively label proteins was demonstrated by monitoring the differential amino acids modification of native horse heart apomyoglobin/holomyoglobin and the human haptoglobin-hemoglobin complex. The obtained data confirmed the Togni reagent-mediated FFAP is an advantageous alternative method for covalent labeling in applications such as protein footprinting and epitope mapping of proteins (and their complexes) in general.
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA19-16084S" target="_blank" >GA19-16084S: Mapping protein surface accessible area utilizing top-down mass spectrometry and reactive radical footprinting</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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