Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00586459" target="_blank" >RIV/61388971:_____/24:00586459 - isvavai.cz</a>
Alternative codes found
RIV/00216224:90127/24:00139172 RIV/00216208:11310/24:10480909
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.3c04161" target="_blank" >10.1021/acs.analchem.3c04161</a>
Alternative languages
Result language
angličtina
Original language name
Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting
Original language description
Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/LX22NPO5107" target="_blank" >LX22NPO5107: National institute for Neurological Research</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Volume of the periodical
96
Issue of the periodical within the volume
19
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
7386-7393
UT code for WoS article
001225426900001
EID of the result in the Scopus database
2-s2.0-85192254871