Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00553502" target="_blank" >RIV/61388971:_____/22:00553502 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0048969721077378?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0048969721077378?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.scitotenv.2021.152659" target="_blank" >10.1016/j.scitotenv.2021.152659</a>
Alternative languages
Result language
angličtina
Original language name
Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
Original language description
Trichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, strain 8M also completely transformed 1,1,2-trichloroethane to vinyl chloride and 1,2-dichloroethane. Quantitative PCR analysis for the 16S rRNA genes confirmed growth of Dehalobacter with TCM and 1,1,2-trichloroethane as electron acceptors. Carbon and chlorine isotope fractionation during TCM transformation was studied in cultured cells and in enzymatic assays with cell suspensions and crude protein extracts. TCM transformation in the three studied systems resulted in small but significant carbon (epsilon(C) =2.7 +/- 0.1 parts per thousand for respiring cells,3.1 +/- 0.1 parts per thousand for cell suspensions, and 4.1 +/- 0.5 parts per thousand for crude protein extracts) and chlorine (epsilon(Cl) =0.9 +/- 0.1 parts per thousand,1.1 +/- 0.1 parts per thousand, and 1.2 +/- 0.2 parts per thousand, respectively) isotope fractionation. A characteristic and consistent dual CCl isotope fractionation pattern was observed for the three systems (combined Lambda(C/Cl) = 2.8 +/- 0.3). This Lambda(C/Cl) differed significantly from previously reported values for anaerobic dechlorination of TCM by the corrinoid cofactor vitamin B12 and other Dehalobacter strains. These findings widen our knowledge on the existence of different enzyme binding mechanisms underlying TCM-dechlorination within the genus Dehalobacter and demonstrates that dual isotope analysis could be a feasible tool to differentiate TCM degraders at field studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science of the Total Environment
ISSN
0048-9697
e-ISSN
1879-1026
Volume of the periodical
813
Issue of the periodical within the volume
MAR 20 2022
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
152659
UT code for WoS article
000748713900012
EID of the result in the Scopus database
2-s2.0-85121909144