Sigma regulatory network in Rhodococcus erythropolis CCM2595
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00555955" target="_blank" >RIV/61388971:_____/22:00555955 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10442590
Result on the web
<a href="https://academic.oup.com/femsle/article-abstract/369/1/fnac014/6528914?redirectedFrom=fulltext#no-access-message" target="_blank" >https://academic.oup.com/femsle/article-abstract/369/1/fnac014/6528914?redirectedFrom=fulltext#no-access-message</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsle/fnac014" target="_blank" >10.1093/femsle/fnac014</a>
Alternative languages
Result language
angličtina
Original language name
Sigma regulatory network in Rhodococcus erythropolis CCM2595
Original language description
The aim of this investigation was to discover the promoters that drive expression of the sig genes encoding sigma factors of RNA polymerase in Rhodococcus erythropolis CCM2595 and classify these promoters according to the sigma factors which control their activity. To analyze the regulation of major sigma factors, which control large regulons that also contain genes expressed under exponential growth and non-stressed conditions, we used the R. erythropolis CCM2595 culture, which grew rapidly in minimal medium. The transcriptional start sites (TSSs) of the genes sigA, sigB, sigD, sigE, sigG, sigH, sigJ, and sigK were detected by primary 5 '-end-specific RNA sequencing. The promoters localized upstream of the detected TSSs were defined by their35 and10 elements, which were identical or closely similar to these sequences in the related species Corynebacterium glutamicum and Mycobacterium tuberculosis. Regulation of the promoter activities by different sigma factors was demonstrated by two independent techniques (in vivo and in vitro). All analyzed sig genes encoding the sigma factors with extracytoplasmic function (ECF) were found to be also driven from additional housekeeping promoters. Based on the classification of the sig gene promoters, a model of the basic sigma transcriptional regulatory network in R. erythropolis was designed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA18-13254S" target="_blank" >GA18-13254S: Stress responses in bacterial degrader of toxic pollutants Rhodococcus erythropolis</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Microbiology Letters
ISSN
0378-1097
e-ISSN
1574-6968
Volume of the periodical
369
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
fnac014
UT code for WoS article
000764887700002
EID of the result in the Scopus database
2-s2.0-85125019957