Fractionation of the water insoluble part of the heterotrophic mutant green microalga Parachlorella kessleri HY1 (Chlorellaceae) biomass: Identification and structure of polysaccharides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00558834" target="_blank" >RIV/61388971:_____/22:00558834 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/22:43925073
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0141813022010820?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141813022010820?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2022.05.108" target="_blank" >10.1016/j.ijbiomac.2022.05.108</a>
Alternative languages
Result language
angličtina
Original language name
Fractionation of the water insoluble part of the heterotrophic mutant green microalga Parachlorella kessleri HY1 (Chlorellaceae) biomass: Identification and structure of polysaccharides
Original language description
The water-insoluble part of Parachlorella kessleri HY1 biomass was subjected to the extraction of cell-wall polysaccharides using polar aprotic solvents (DMSO, LiCl/DMSO) and aqueous alkaline solutions (0.1, 1 and 4 mol.l(- 1) of NaOH). Proteins predominated in all the crude extracts and in the insoluble residues were partially removed by treatment with proteolytic enzymes (pepsin and pronase), and in some cases with the HCl/H2O2 reagent, yielding purified polysaccharide-enriched fractions. These treatments led to the solubilisation of some products in water. The composition and structure of isolated polysaccharides were characterised based on monosaccharide composition, glycosidic linkage and spectroscopic analyses. The DMSO extract contained mainly proteins, and polysaccharides were not detected. The water-soluble parts isolated from the LiCl/DMSO extract contained alpha-L-rhamnan, alpha-D-glucan and beta-D-glucogalactan, the water-insoluble part contained (1> 4)-beta-D-xylan, first isolated from the biomass of green microalgae. The alkali extracts contained polysaccharides of similar structure, and also water-insoluble (1> 4)-beta-D-mannan. The insoluble part after all extractions contained alpha-chitin as the main polysaccharide, which was confirmed by spectroscopic methods. All these polysaccharides can play a certain role in the cell wall structure of this microalga.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/FV10155" target="_blank" >FV10155: Algal Biotechnology for Food - preparation of proteins and carotenes by heterotrophic cultivation of chlorophyl-deficient clones of microalgae</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Biological Macromolecules
ISSN
0141-8130
e-ISSN
1879-0003
Volume of the periodical
213
Issue of the periodical within the volume
July 31
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
27-42
UT code for WoS article
000812321000004
EID of the result in the Scopus database
2-s2.0-85131083302